Software⏱️ 2 min read📅 2026-05-31

How to Fix: Could not load file or assembly or one of its dependencies. Access is denied. The issue is random, but after it happens once, it continues

Random access denied error on one server, resolves after recompilation and web.config modification.

Quick Answer: Check permissions for the IIS application pool and website, ensure that the .NET framework version is consistent across all servers.

The 'Could not load file or assembly or one of its dependencies. Access is denied' error can be a frustrating issue to deal with, especially when it's random and persistent. In your scenario, the problem seems to be isolated to one server out of six production servers, which makes it even more puzzling.

⚠️ Common Causes

  • Permission issues: The issue might be related to the permissions set on the server or specific files/directories.

✅ Best Solutions to Fix It

Method 1: Application Pool Identity

  1. Step 1: Set the correct application pool identity for your site. Ensure that the identity has the necessary permissions to access the required files and directories.

Method 2: File Permissions

  1. Step 1: Check the file permissions on the server. Ensure that the site's application directory and any dependent files/directories have the correct permissions (e.g., read, write, execute).

💡 Conclusion

In conclusion, fixing the 'Could not load file or assembly or one of its dependencies. Access is denied' error requires a combination of troubleshooting and configuration adjustments. By following these steps, you should be able to identify and resolve the issue on the affected server.

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